ČSN EN ISO 6579-4   (560088) Mikrobiologie potravinového řetězce - Horizontální metoda průkazu, stanovení počtu a sérotypizace bakterií rodu Salmonella - Část 4: Identifikace monofázické Salmonella Typhimurium (1,4,[5],12:i:-) pomocí polymerázové řetězové reakce (PCR)

Anotace textu normy ČSN EN ISO 6579-4   (560088)

This document specifies a horizontal in vitro method for the molecular identification and differentiation of the monophasic variant of Salmonella enterica subsp. enterica serovar Typhimurium (1,4,[5],12:i:-) lacking the second H phase H:1,2, starting from isolates. The method detects specific DNA sequences of an intergenic region of the first H phase flagellin cluster for identification of Salmonella enterica subsp. enterica serovar Typhimurium (further called Salmonella Typhimurium) and specific DNA sequences of genes associated with second H phase flagellar antigen expression.

The method is applicable for:

- differentiation of the isolate under analysis between monophasic Salmonella Typhimurium and the monophasic variant of another Salmonella on-Typhimurium serovar that has the same antigenic formula,

- identification of the isolate under analysis being either monophasic Salmonella Typhimurium or (biphasic) Salmonella Typhimurium.

This document is applicable for the analysis of a pure culture belonging to the genus Salmonella, isolated from:

- products intended for human consumption,

- products intended for animal feeding,

- environmental samples in the area of food and feed production and handling,

- samples from the primary production stage.

This document can also be applied in other domains for identification of monophasic Salmonella Typhimurium (e.g. environmental, human health, animal health).